LOCUS       40924_23579       12257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB652 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12257)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1891
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    1901..2025
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2062..2092
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2146..2802
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3147..3449
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3493..3617)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3643..3895
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3929..3945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3953..3969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3977..4007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4022..4043)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4079..4331)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4494..5042)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(5094..5273)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5779..5803)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6324..7112
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7361..7949
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8135..8275)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8619..8813)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8882..9952)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10384..11010)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg agtaaaggag aagaactttt
     1201 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     1261 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     1321 cactactgga aaactacctg ttccatggcc aacacttgtt actactttcg cttacggtgt
     1381 gcagtgcttc tcaagatacc cagatcatat gaagcggcac gacttcttca agagcgccat
     1441 gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac
     1501 acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat
     1561 cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact tcaactccca
     1621 caacgtgtac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
     1681 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
     1741 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1801 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1861 gattacacat ggcatggatg aactatacaa agctgttatc acaagtttgt acaaaaaagc
     1921 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     1981 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc ccctcgaggg
     2041 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2101 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2161 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     2221 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2281 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2341 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     2401 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2461 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2521 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     2581 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2641 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2701 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2761 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc
     2821 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     2881 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     2941 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3001 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3061 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3121 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3181 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3241 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     3301 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     3361 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     3421 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     3481 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     3541 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     3601 ttcttgtaca aagtggtgat aacctagagc tcgaatttcc ccgatcgttc aaacatttgg
     3661 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     3721 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     3781 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     3841 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     3901 ttggttccgg aaccaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4021 aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga
     4081 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     4141 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     4201 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     4261 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     4321 tttgaacgat cggggaaatt cgagctgggg acgtccccag ctcggtaccc cctcctgggc
     4381 acggtcgtac gcgatccgcc gcggttccac ctcaccattg cgggtatgcc agttgagttc
     4441 gatgagttcc cgtaccagct ccaactcggg cgatgccacg cgcactccgt tcagatctcg
     4501 gtgacgggca ggaccggacg gggcggtacc ggcaggctga agtccagctg ccagaaaccc
     4561 acgtcatgcc agttcccgtg cttgaagccg gccgcccgca gcatgccgcg gggggcatat
     4621 ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca gcccgatgac agcgaccacg
     4681 ctcttgaagc cctgtgcctc cagggacttc agcaggtggg tgtagagcgt ggagcccagt
     4741 cccgtccgct ggtggcgggg ggagacgtac acggtcgact cggccgtcca gtcgtaggcg
     4801 ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga cctcgccgtc cacctcggcg
     4861 acgagccagg gatagcgctc ccgcagacgg acgaggtcgt ccgtccactc ctgcggttcc
     4921 tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt agtggttgac gatggtgcag
     4981 accgccggca tgtccgcctc ggtggcacgg cggatgtcgg ccgggcgtcg ttctgggctc
     5041 atatcttatt gccccccggg atcagatctg gattgagagt gaatatgaga ctctaattgg
     5101 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     5161 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     5221 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
     5281 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     5341 ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     5401 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     5461 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     5521 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     5581 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
     5641 cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc cgtcgagatt aatagaaata
     5701 aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     5761 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     5821 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     5881 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     5941 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     6001 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     6061 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     6121 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     6181 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     6241 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     6301 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac
     6361 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     6421 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     6481 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     6541 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     6601 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     6661 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     6721 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     6781 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     6841 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     6901 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     6961 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     7021 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     7081 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc
     7141 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     7201 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     7261 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7501 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8101 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     8161 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     8221 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     8281 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8341 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     8401 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     8461 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     8521 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     8581 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     8641 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     8701 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     8761 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     8821 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     8881 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     8941 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     9001 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     9061 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     9121 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     9181 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     9241 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
     9301 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
     9361 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
     9421 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
     9481 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
     9541 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
     9601 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
     9661 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
     9721 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
     9781 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
     9841 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
     9901 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
     9961 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
    10021 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
    10081 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
    10141 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
    10201 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
    10261 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    10321 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
    10381 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
    10441 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
    10501 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
    10561 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
    10621 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
    10681 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
    10741 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    10801 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    10861 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    10921 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    10981 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    11041 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    11101 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    11161 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    11221 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    11281 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    11341 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    11401 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    11461 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    11521 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    11581 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    11641 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    11701 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    11761 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    11821 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    11881 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    11941 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    12001 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    12061 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    12121 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    12181 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    12241 gacgaacgga taaacct
//
